Isolation, Purification and Structural Elucidation of Flavonoids from Methanol Extract of Ariel Parts of Derris Brevipes (benth.) Baker

Two flavonoids were isolated by performing column chromatographic separation of methanol extract of ariel parts of Derris brevipes (Benth) Baker. Structural elucidation of purified compounds was done on the basis of spectral analysis using IR, 1 H NMR, 13 C NMR 2D NMR spectroscopy and Mass spectrometry. The structures of the flavonoids were elucidated as 2’, 4’-dihydroxy-4-methoxy-3’prenylchalcone (compound1) and Leutolin-3'-O-xilofuranose (compound 2). INTRODUCTION: The genus Derris of the family Leguminosae, tribe Tephrosieae, subfamily Papilonoidae, has recieved much attention from phytochemical perspective as this species of this tribe is known for its large content of flavonoids 1 . Derris species are distributed over the tropical areas of asia and east africa. All species are woody perennials and mostly climbers. Abundant flavonoids and many other compounds of phytochemical interest are isolated from Derris plants and can be categorized into aurones, chalcones, coumarins, flavans, flavanones, flavones, glycosides, isoflavones, pterocarpans, quinoids, rotenoids, steroids, stilbenes and terpenoids. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.6(1).491-01 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/IJPSR.0975-8232.6(1).491-01 Many kinds of Derris flavonoids possess wide varieties of biological activities. Derris is popular ichthyotoxic 2 , insecticide 3 , pesticides 4 , molluscide 5 , antiviral 6 and have antibacterial 7 properties. Derris is also used as analgesic 8 , antipyretic 9 , for arthistic symptoms 10 , antidysenteric 11 , antidiuretic 12 , antispasmodic 13 , counter-irritant 14 , for purification of blood 15 , for treatment of leprosy 16 , sore throat 17 and rheumatism 18 . Derris species from India are known for medicinal value and different parts of species have been used in folk medicine for bronchitis 19 , cough, rheumatoid arthritis 20 , diabetes and as antifertility agent 21 Derris brevipes is a climbing shrub, distributed widely in India. Literature review revealed that chemical investigation of the aerial part of Derris brevipes has not been carried out. To discover naturally occurring bioactive compounds from Derris brevipes the methanol extract of ariel parts was column chromatographed. Column chromatographic separation resulted in flavonoids.